Table 2.
Mutation | Sequence of the primers | mp | Product length |
---|---|---|---|
C61G |
F: 5’ CTC TTA AGG GCA GTT GTG AG 3’ |
59,1°C |
158 pz |
R: 5’ ATG GTT TTA TAG GAA CGC TAT G 3’ | 58,6°C | 118 pz |
Mutation | Sequence of the primers | mp | Product length |
---|---|---|---|
C61G |
F: 5’ CTC TTA AGG GCA GTT GTG AG 3’ |
59,1°C |
158 pz |
R: 5’ ATG GTT TTA TAG GAA CGC TAT G 3’ | 58,6°C | 118 pz |