TABLE 1.

Phenotypic analysis of unfractionated PBMC after 7 days in culture^a

Cow	% of cells (mean ± SEM) in cell typeb
	γδ T cells	CD4+ cells	CD8α+ cells	PBMC
				MHC-II+ B cell+	MHC-II+ B cell−	MHC-II− B cell+
Healthy (n = 4)	20.09 ± 6.81 A	28.23 ± 2.71	24.63 ± 0.84	14.38 ± 2.22 A	17.88 ± 5.18	4.32 ± 2.12 A
Subclinically infected (n = 2)	27.25 ± 0.25 A	33.70 ± 9.70	19.65 ± 4.85	14.82 ± 4.98 A	7.51 ± 1.05	5.05 ± 3.06 A
Clinically infected (n = 2)	14.80 ± 1.00 B	18.00 ± 6.30	10.72 ± 7.18	36.45 ± 1.95 B	9.39 ± 4.91	15.30 ± 3.00 B

^aPhenotypic analyses were performed 18 h after the replacement of the supernatant in culture with fresh medium and before the addition of M. avium subsp. paratuberculosis to the in vitro cultures.

^bThe different letters after the values indicate statistically significant differences (P < 0.05) (values with the same letter were not significantly different).