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. 2013 Aug 23;8(8):e72687. doi: 10.1371/journal.pone.0072687

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© 2013 Shevade et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) P . yoelii cell fractionation scheme. Various fractions used for analysis are marked in bold. Proteins were separated on 10% SDS PAGE.

(B) Western blot of FV proteome probed with (a) anti-rPfeno (mouse) and anti-Ub (mouse) antibodies. Note that three variants of enolase were observed while two of these (65 & 75 kDa) are ubiquitinated (dotted line box). Matched amounts of proteins were loaded in both the lanes.

(C) Antibody pull-down assays for different sub-cellular fractions using anti-rPfeno (mouse) and anti-Ub antibodies (mouse). Blots of pull-down proteins were probed with anti-rPfeno (rabbit) to detect the presence of enolase.

(D) Western blots of whole cell soluble fraction and total FV probed with ant-PfP0 (cytosolic marker) and anti-falcipain-2 (FV marker) antibodies. Equivalent amount of total proteins were loaded in each lane. Note the enrichment of falcipain-2 and near absence of P0 in FV preparation indicating that preparation used in above experiments is highly enriched in FV.