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Virulence and nodulation complementation assays. (A) Alfalfa plants inoculated with different strains of S. meliloti. (1 and 2) F34; (3 and 4) LI1; (5 and 6) LI1 (pBB4cgt); (7 and 8) noninoculated control. (B) Kalanchoe leaf inoculated with different strains of A. tumefaciens. The virulence and nodulation assays were carried out as described in Materials and Methods. Photos were taken 4 weeks postinoculation in both assays.
