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Parasite-specific antibodies in the serum of C3H/HePAS, A/J, F1 (A/J × C3H/HePAS), and F2 (A/J × C3H/HePAS) chronically infected mice and in noninfected age-matched C3H/HePAS and A/J controls. Mice from the F1 and F2 generations, as well as from parental controls, were infected with 106 Sylvio X10/4 trypomastigotes. At day 200 after infection, mice were bled and their sera were analyzed by ELISA with T. cruzi antigen to determine the level of specific IgG2a and IgG1 antibodies.
