Figure 2. Effects of TRß2 and T3 on human red opsin promoter activities.

Luciferase plasmid was transfected into HEK293T cells with TRß2 expression vector or a control vector, and cells were harvested after 28 hours of incubation. Luciferase activities were examined and expressed as relative values. A. Luciferase plasmid containing 5′ proximal promoter region was transfected with TRß2 expression plasmid or control vector. B–D. Luciferase plasmids containing 5′ promoter region (B), exon/intron region fused with 1.0 kb proximal promoter region (C), or p55Bluc vector (D) was transfected into HEK293T with TRß2 expression plasmid or a control vector, and cultured for 12 hours, then cultured additional 16 hours in the presence or absence of T3 (100 nM). Luciferase activity was examined and expressed as relative values. Pink colored bars indicate samples transfected with TRß2, and dark grey and dark pink show those with stimulation by T3. p value; **<0.01, *<0.05, or actual value was calculated by Student’s t-test.