FIG. 1.
Antiglycan IgM antibodies in normal human serum. Forssman and blood group A glycolipids (lane 1, upper and lower bands, respectively), and gangliosides GA1, GM1, GD1a, and GD1b (lane 2, top to bottom) were separated by HPTLC as described in Materials and Methods. Plates were incubated with the stated human serum, and after being washed, IgM antibody binding was detected with peroxidase-labeled anti-human IgM antibodies. One plate was stained with orcinol reagent for chemical detection of glycolipids.