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. 2013 Aug 8;13:114. doi: 10.1186/1471-2229-13-114

Figure 3.

Figure 3

Expression patterns of the nine ZmZIP genes in maize seedlings under various metal conditions. Two-week old shoots (S) and roots (R) of maize seedlings, under standard nutrient condition (CK), Zn, Fe-deficiency (Zn-), (Fe-), 200 μM ZnSO4 (Zn++) and 500 μM FeSO4 (Fe++) treated, were harvested respectively at 0 h, 6 h, 12 h, 24 h, 48 h, 96 h after treatment. Relative mRNA abundance of each gene was normalized with ZmActin1 gene. Data from real-time RT-PCR experiments were analyzed according to the 2-∆∆Ct method. The error bars indicate standard deviations.