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. 2004 Apr;72(4):2288–2302. doi: 10.1128/IAI.72.4.2288-2302.2004

FIG. 3.

FIG. 3.

Immunoblot analysis of His-tagged EspI in cell pellets and concentrated supernatants. Equivalent amounts of His-tagged EspI were detected in cell pellets of wild-type, Tn::escD (P10G3), and ΔescF (ICC176) strains (lanes 5, 6, and 7). His-tagged EspI was also detected strongly in concentrated supernatant of the wild-type strain (lane 1), but no His-tagged EspI was detected in the equivalent amount of concentrated supernatant of Tn::escD strain P10G3 (lane 2) and only a trace amount was detected in the supernatant of ΔescF strain ICC176 (lane 3). Lane 4 was left empty to prevent overspill between wells. The position of a protein corresponding to His-tagged EspI (47.2 Da) is indicated by arrows.