Skip to main content
. 2004 Apr;72(4):1991–2001. doi: 10.1128/IAI.72.4.1991-2001.2004

FIG. 6.

FIG. 6.

Ratios of IFN-γ, IL-12 p70, and IL-12 p40 levels observed for DC populations isolated from infected versus naïve BALB/c and C57BL/6J lymph nodes at 3, 6, 9 (IFN-γ only), and 12 weeks postinfection with 103 L. major V121 metacyclic promastigotes. Twenty-four to 50 mice were used per time point. For the evaluation of IFN-γ, cells were stimulated in vitro with murine rIL-12 and rIL-18 for 72 h. For IL-12 cytokine production, cells were incubated for 24 h with murine recombinant granulocyte-macrophage colony-stimulating factor, rIL-4, rIFN-γ, and CpG, as described in Materials and Methods. All stimulations were done in duplicate or triplicate. For some time points, two independent experiments were performed, with all results being similar. Data are shown for IFN-γ production in BALB/c (A) and C57BL/6J (D) mice; IL-12 p70 production in BALB/c (B) and C57BL/6J (E) mice; and IL-12 p40 production in BALB/c (C) and C57BL/6J (F) mice. (−/−), double-negative (CD8− Dec-205−) cells; CD8H+, CD8hi Dec-205int cells; DDC, CD8int Dec-205int cells; LC, CD8int Dec-205hi cells.