Figure 8.

The pH dependence of the LF:PA₆₃ channel binding constant as estimated from the channel conductance at V = +70 mV. The symbols correspond to the pH conditions described in Fig. 3 (pH_c|t 7.2|7.2 □; pH_c|t 5.5|7.2 ○). The solid lines are the least-squares best fits of a simple binding equation for 1:1 stoichiometry (i.e., 1/(1 + [LF]/K_d), where K_d is the reaction dissociation constant. (Inset) The pH dependence of K_d. The pH in the trans chamber was 7.2. The dashed line is to guide the eye. The error bars represent the standard deviation from n = 3–5 membranes.