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Changes in Johnin PPD-induced proliferation by whole-blood cells from M. avium subsp. paratuberculosis-infected (•) and uninfected (○) cattle in the presence of an IL-10-neutralizing MAb. Whole blood diluted 1/10 was stimulated with 5 μg of Johnin PPD/ml, and after 48 h of stimulation, the cells were pulsed with 0.5 μCi of [3H]thymidine and incubated for an additional 24 h. Radioactivity was measured with a liquid scintillation counter.
