FIG. 5.

Changes in IL-10 mRNA expression by whole blood from M. avium subsp. paratuberculosis-infected (•) and uninfected (○) cattle after stimulation with Johnin PPD. RNA was purified from whole blood by use of the QIAamp kit and reverse transcribed using the TaqMan reverse transcription reagents. The resulting cDNA was amplified for IL-10 and glyceraldehyde-3-phosphate dehydrogenase by real-time PCR with the TaqMan Universal PCR Mastermix (Applied Biosystems). Results are expressed as IL-10 mRNA expression of PPD-stimulated blood as a percentage of that in unstimulated whole blood.