TABLE 2.
Primer | Sequencea |
---|---|
−496 hilA | 5′ GTCGACCAGATGACACTATCTCCTTCC 3′ |
+350 hilA | 5′ GAATTCATAATAGTGTATTCTCTT 3′ |
5′ hilD | 5′ GTCGACGGATAATAGGTATCCAGCCAG 3′ |
3′ hilD | 5′ GAATTCGTTATTTTAATGTTCCTT 3′ |
5′ sicA | 5′ GTCGACATGGCTGACTACAGTTTG 3′ |
3′ sicA | 5′ GAATTCCATTACTTACTCCTGTTATCT 3′ |
5′ hilC | 5′ GTCGACAAGGTGATACCGGGTACGATT 3′ |
3′ hilC | 5′ GAATTCATCCTGTGTGCTATAAGGAAC 3′ |
5′ ssrA | 5′ GTCGACCCACTAAATGTAGCTGTT 3′ |
3′ ssrA | 5′ GAATTCAATGCTTCCCTCCAGTTG 3′ |
5′ rpsU | 5′ GTCGACGGCACTACGCCGCCGTAGTCA 3′ |
3′ rpsU | 5′ GAATTCGCATGTGCCTCTCACCTTTGA 3′ |
EcoRI and SalI sites are underlined.