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. 2004 Apr;72(4):2002–2013. doi: 10.1128/IAI.72.4.2002-2013.2004

TABLE 2.

Sequences of primers used in this work

Primer Sequencea
−496 hilA 5′ GTCGACCAGATGACACTATCTCCTTCC 3′
+350 hilA 5′ GAATTCATAATAGTGTATTCTCTT 3′
5′ hilD 5′ GTCGACGGATAATAGGTATCCAGCCAG 3′
3′ hilD 5′ GAATTCGTTATTTTAATGTTCCTT 3′
5′ sicA 5′ GTCGACATGGCTGACTACAGTTTG 3′
3′ sicA 5′ GAATTCCATTACTTACTCCTGTTATCT 3′
5′ hilC 5′ GTCGACAAGGTGATACCGGGTACGATT 3′
3′ hilC 5′ GAATTCATCCTGTGTGCTATAAGGAAC 3′
5′ ssrA 5′ GTCGACCCACTAAATGTAGCTGTT 3′
3′ ssrA 5′ GAATTCAATGCTTCCCTCCAGTTG 3′
5′ rpsU 5′ GTCGACGGCACTACGCCGCCGTAGTCA 3′
3′ rpsU 5′ GAATTCGCATGTGCCTCTCACCTTTGA 3′
a

EcoRI and SalI sites are underlined.