TABLE 3.
Reporter expression after infection of epithelial cells
| Reporter | % GFP-positive bacteria at indicated times (h)a
|
Decrease (n-fold)
|
||||
|---|---|---|---|---|---|---|
| 0b | 2.5 | 5 | 24 | 0-24 h | 2.5-24 h | |
| hilA-gfp | 56.6 ± 22.9 | 30.8 ± 11.5 | 20.4 ± 9.8 | 13.2 ± 11.2 | 4.3 | 2.3 |
| sicA-gfp | 43.8 | 31.2 ± 5.2 | 12.2 ± 6.6 | 2.5 ± 1.3 | 17.5 | 12.5 |
| hilD-gfp | 67.2 ± 18.8 | 29.1 ± 11.6 | 22.3 ± 8.88 | 17.2 ± 10.2 | 3.9 | 1.7 |
| hilC-gfp | 76.9 ± 14.1 | 14.9 ± 4.9 | 12.9 ± 7.8 | 8.8 ± 4.9 | 8.7 | 1.7 |
| ssrA-gfp | 70.7 ± 19.9 | 37.2 ± 12.4 | 30.5 ± 13.9 | 11.2 ± 7.2 | 6.3 | 3.3 |
| rpsU-gfp | 76.1 ± 30.6 | 49.7 ± 9.1 | 33.6 ± 5.0 | 29.2 ± 7.5 | 2.6 | 1.7 |
a
Average and standard deviation for GFP-positive bacteria before (0) or after infection for at least three separate experiments (0 time point for sicA was tested only one time).
b
Average for GFP-positive bacteria within the inoculum before infection of HEp-2 cells.