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. Author manuscript; available in PMC: 2015 Jun 5.
Published in final edited form as: Eur J Pharmacol. 2012 Jun 23;732:159–168. doi: 10.1016/j.ejphar.2012.06.001

Fig. 3.

Fig. 3

Effect of HEPES on acetylcholine concentration/response curves. Acetylcholine concentration/response kinetics were determined in Phosphate ND-96 buffer containing five different concentrations of HEPES. (A) Response traces obtained from a single oocytes expressing the HS α4β2 preparation in phosphate ND-96 buffer at increasing acetylcholine (ACh) concentrations (0.1 μM–1 mM) either in the absence of HEPES (trace a) or co-applied with 100 μM HEPES (traces b–g). (B) Acetylcholine concentration/response curves obtained using the HS receptor preparation in the presence of 0 μM, 2.5 μM, 7.5 μM, 22.5 μM or 100 μM HEPES (potentiating concentrations). Individual response amplitudes were normalized to the peak response obtained on exposure to 10 μM acetylcholine alone. Each point represents the composite data obtained from four different experiments on four different oocytes from at least 2 different frogs. Individual pEC50 values are given in the text.