FIG. 5.

IL-8 mRNA expression induced by LPS is inhibited by NAC and DMSO. THP-1 cells were incubated with various antioxidants and then treated with 10 μg of LPS per ml for 4 h. Total RNA was extracted and reverse transcribed. IL-8 mRNA expression was assessed by real-time PCR. Data are expressed as the means ± standard error of the mean of four (NAC and DMSO) or three (all other samples) independent experiments carried out in duplicate. Statistical differences between LPS alone and LPS plus antioxidant samples are indicated; *, overall P value of ≤0.05, as determined with the Wilcoxon test with an adjusted Bonferroni correction.