TABLE 1.
Oligonucleotide primers used for quantitative real-time PCR
| ORFa | Primer sequence (5′-3′)
|
|
|---|---|---|
| Forward | Reverse | |
| 16S | CACGCAGTGTCGCTCCG | TACGGGAGGCAGCAGCTAAG |
| BB0011 | AAGAGCCGCTTGGACATTCG | ATGCCTGCTATTTTAGAAAGAGTG |
| BB0057 | CTGTGCTCAGGAAGGCATCAGAAAC | CAAGACCATCAACAATAGAAGAATG |
| BB0143 | ATGAACATTTTTAAAATTTTA | TTTGATTAATCTTTTTTTAAATTC |
| BB0147 | GCTTCTGATGATGCTGCTGG | ATGTGCCGTTACCTGATTGAACTGCC |
| BB0369 | TGTGGGTGCTTGTCAAAAAAAG | GGCAGATTTAGTATTTACAGC |
| BB0377 | CATACAAAACTCAACCCTGGC | CGGTCCAAACTTCATTATTTCTAAG |
| BB0516 | ATGACAGTGACAAACATAAACAACAC | AGTGAGTCTATTTTTCCGCTGG |
| BB0603 | CAACGATTTATTGAGCCCAAC | CGCTGCTTTTGAGATGTGTCC |
| BBA30 | AGCGACCTGGCAGAAGAAAGCACTAC | CTGTGGTGGGAATCAATAAAAGAAC |
| BBB19 | GAGGTTGAAGCGTTGCTGTC | TCCATTGTGATTATTTTCGGTATC |
| BBD18 | GGGGGCATAAAAGGAAC | CACCGGTTTGCATATTAAC |
| BBI07 | GTGCAGCTACTAAAAGGCGCT | ATGTAACTTAATTAAGTTTTTAAT |
| BB131 | ATACAGATGATGAAACAAGAGC | TAAACGATAGTTGGAATCCGC |
| BBI39 | TGAAAAAGAAGCCGAAAAGTTG | GTAAGCGTCAATGGTTGCG |
| BBM35 | CTGGAGTTGAGTTTGATTGG | GGCTTTTCTCTTTTTTTATTTTCG |
| BBP08 | GTGTGGGGGTTGTCTAATG | TTCGGTCTTTTGGTTCATAG |
| BBP10 | GGGTTTAAGGCATATGCAAC | TACACCGGCTCTATAATTATG |
| BBP13 | CGAATTCTATGTGATTTTGGAT | CAAAAGCAACAAAATCACCAAC |
| BBP23b | ATGGATACTATTAAATTAACCG | ATCTCTTTTTTTAATGTGATTTT |
| BBP24c | AAGGATTCTTTTTGGTTTATG | AGTGCCGCATTTATTGCTGG |
| BBP25 | GTTTAGGACTTAATTTACTATC | GTCAGTAGAGATAGCCTC |
| BBT03 | CGATACACTGGTCGCAAGAATAAC | GGGACATTTTACGATTACTTTTGG |
a
Boldface ORFs represent genes used for real-time PCR concordance data.
b
blyA primers recognize all B. burgdorferi B31 paralogy alignment GBB family 109 members due to their 100% identity.
c
blyB primers recognize all B. burgdorferi B31 paralogy alignment GBB family 111 members due to their high conserved homology.