Figure 2.

H10 and F10A oligonucleotides interact in vitro with embryonic and adult striatal nuclear proteins. A, EMSA shows that ³²P-labeled F10A and H10 oligonucleotides bind with proteins in embryonic (E16–17) and adult striatal nuclear extracts. The specificity of the complexes was determined by the addition of 100-fold excess of unlabeled F10A or H10 oligonucleotide as a competitor. * Refers to nonspecific complexes, i.e., not quenched by competitor. B, EMSA reveals the formation of complexes between H10 and nuclear extracts from adult striatum, hippocampus, cortex, cerebellum, and brainstem.