TABLE 2.
RT-PCR primers
| Gene | Forward primer sequence (5′ - 3′) | Reverse primer sequence (5′ - 3′) | Expected size (bp) |
|---|---|---|---|
| ureA (HP0073) | ATGAAACTCACCCCAAAAGAG | CTCCTTAATTGTTTTTACATA | 714 |
| lpxD (HP0196) | ATGAAATTAAGCGAATTGTTA | TTAAGATTTAAAAAACCCTTT | 1,011 |
| omp6 (HP0229) | ATGAAAAAAACGATTTTACTT | TCAATAGGCCAAAACATAATT | 1,452 |
| secG (HP1255) | GTGTTTATGACAAGCGCTCTG | TTATTTTTCATCCTTTTTTTG | 606 |
| fabH (HP0202) | ATGGAATTTTACGCCTCTCTT | CTAACTTCCTCCAAAATACAC | 996 |
| HP1285 | ATGAGTGTTTTAAATGCCAAA | TTATTTTTTATTCTGCCATGC | 693 |
| HP0222 | ATGGAAAAGACAGAAAACACA | TTACTCTATTTTTCTTAAAGC | 222 |
| HP0836 | ATGCCCATGCGTTTGCACACT | CTAGAGCCAGGGCTTGAGAGG | 360 |
| omp11 (HP0472) | ATGATTAAAAGAATTGCTTGT | TTAGAAAGTAAAGACATAATC | 561 |