Figure 4.

Arrestin movement in response to light in P21 WT and Rs1–KO mice. In the dark, arrestin immunostaining was confined to the OPL, ONL, and IS in WT mice. After exposure to light intensity of 1 sc.cd/m² for 1 h, arrestin was concentrated in the IS compartment, and very sparse staining was detected in the ROS. Light of 2 sc.cd/m² was able to mobilize arrestin from the IS to the ROS. In Rs1–KO retina, the threshold light intensity for arrestin translocation was the same as WT retina. However, at light intensity below the threshold, arrestin redistribution in the inner retina was confined mainly in the ONL and OPL, and none was detected in the IS of Rs1–KO mice. Scale bar, 15 μm.