Figure 2.

Types and distribution of phosphorylated tau-immunoreactive neurons in CA1 region after 6 days of ZPAD treatment. Shown is a vertical section that extends across most of the basal (stratum oriens), and the inner third of the apical (stratum radiatum), dendritic fields in field CA1 of a cultured slice that had been exposed to ZPAD for 6 days. The majority of the AT8-immunopositive cells are found in the basal dendritic field. The cell bodies (stratum pyramidale) and apical dendrites of the pyramidal cells, by far the most numerous population of neurons in the section, are with few exceptions unlabeled. The stained elements are not homogeneous. The cells marked as 1 appear to be intact neurons with immunopositive processes and dense deposits accumulating within the cell body. The labeled neuron marked as 2 has swollen and distorted dendrites. The elements marked as 3 appear to be remnants of neurons. (Bar = 50 μm.)