Increased bacterial loads after infection of TLR2−/− mice with L. monocytogenes. TLR2+/+ (black bars), TLR2−/− (white bars), and MyD88−/− (hatched bars) mice were infected with 2 × 105 CFU of Listeria i.v. and sacrificed at day 2 postinfection, and the total numbers of CFU per liver (A) and spleen (B) were determined. Results are from one representative experiment of two independent experiments. Results are expressed as means ± SD. *, P < 0.05, **, P < 0.01.