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. 2013 Aug 20;105(4):880–886. doi: 10.1016/j.bpj.2013.07.019

Figure 1.

Figure 1

DhPIEZO1 does not inactivate. (A) Whole-cell currents of WT hPIEZO1 rapidly inactivate. (B) Whole-cell currents of DhPIEZO1 as a function of indentation depth showed no inactivation. Note the slow rate of deactivation after the stimulus is removed. (C) WT channels are sensitive to hypotonic swelling. Prior to swelling, there was less current for the same incremental indentation than after swelling. Also shown is the response of DhPIEZO1 prior to swelling. (D) Comparison of DhPIEZO1 responses before and after hypotonic swelling with 50% osmolarity. The inset shows the same data normalized. The sensitivity to indentation is similar, suggesting that the channels are already near a saturated level of stress in the resting state. (E) Deactivation is extremely slow and voltage independent. Deactivation for this channel may represent the kinetics of domain reformation rather than channel kinetics. (F) The I/V curve of peak currents shows a reversal potential near 0 mV for DhPIEZO1, indicating that the mutations do not affect the pore.