TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| Strains | ||
| E. coli | ||
| DH5α | F− ϕ80d lacZΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 hsdR17(rK− mK+) phoA supE44λ−thi-1 gyrA96 relA1 | GIBCO BRL |
| SM10λpir | thi thr leu tonA lacY supE recA::RP4-2-Tc::Mu λpirR6K | 28 |
| B. pertussis | ||
| BP536 | Wild-type, nalidixic acid-resistant, streptomycin-resistant derivative of Tohama I | 31 |
| BP536Δptxptl936-8003 | BP536 with an in-frame deletion in the ptx-ptl region, from nucleotide 936 to 8003 | This study |
| BP536Δptxptl936-3941 | BP536 with an in-frame deletion in the ptx-ptl region, from nucleotide 936 to 3941 | This study |
| BP536Δptxptl3822-8003 | BP536 with an in-frame deletion in the ptx-ptl region, from nucleotide 3822 to 8003 | This study |
| BP536::pAMC48 | BP536 containing a ptxS1′-phoA fusion | This study |
| BP536::pAMC17 | BP536 containing a ptxS2′-phoA fusion | This study |
| BP536::pAMC67 | BP536 containing a ptlA′-phoA fusion | This study |
| BP536::pAMC107 | BP536 containing a ptlB′-phoA fusion | This study |
| BP536::pAMC121 | BP536 containing a ptlD′-phoA fusion | This study |
| BP536::pAMC28 | BP536 containing a ptlF′-phoA fusion | This study |
| BP536Δptxptl936-8003::pAMC28 | BP536Δptxptl936-8003::pAMC28 containing a ptlF′-phoA fusion | This study |
| BP536Δptxptl936-3941::pAMC28 | BP536Δptxptl936-3941::pAMC28 containing a ptlF′-phoA fusion | This study |
| BP536Δptxptl3821-8003::pAMC28 | BP536Δptxptl3821-8003::pAMC28 containing a ptlF′-phoA fusion | This study |
| Plasmids | ||
| pUFR047 | Broad-host-range (IncW) vector; Mob+lacZα+; gentamicin-resistant | 7 |
| pGEM11Zf(+) | Ampicillin-resistant (Ampr) cloning vector | Promega |
| pALTER-1 | Tetr cloning vector | Promega |
| pUC19 | Ampr cloning vector | GIBCO BRL |
| pZErO-1 | Zeocin-resistant cloning vector | Invitrogen |
| pSZH4 | pUC19 containing the entire ptx-ptl region | 12 |
| pSS1129 | Genr Ampr Sms allelic exhange vector | 30 |
| pAMC48 | pSS1129 containing nucleotides 1 to 509 of the ptx-ptl region followed by phoA devoid of its initiation codon | This study |
| pAMC17 | pSS1129 containing nucleotides 1 to 1358 of the ptx-ptl region followed by phoA devoid of its initiation codon | This study |
| pAMC67 | pSS1129 containing nucleotides 1312 to 3686 of the ptx-ptl region followed by phoA devoid of its initiation codon | This study |
| pAMC107 | pSS1129 containing nucleotides 2831 to 4013 of the ptx-ptl region followed by phoA devoid of its initiation codon | This study |
| pAMC121 | pSS1129 containing nucleotides 5738 to 6806 of the ptx-ptl region followed by phoA devoid of its initiation codon | This study |
| pAMC28 | pSS1129 containing nucleotides 5007 to 9056 of the ptx-ptl region followed by phoA devoid of its initiation codon | This study |
| pAMC111 | pUFR047 containing the nine ptl genes under the control of the ptx-ptl promoter | This study |
| pAMC147 | pUFR047 containing ptlA, ptlB, and ptlC under the control of the ptx-ptl promoter | This study |
| pTC11 | pUFR047 containing ptlD, ptlI, ptlE, and ptlF under the control of the lac promoter | This study |
| pAMC151 | pUFR047 containing ptlI-ptlH under the control of the lac promoter | This study |
| pAMC171 | pUFR047 containing ptlA under the control of the ptx-ptl promoter | This study |
| pAMC176 | pUFR047 containing ptlB and ptlC under the control of the ptx-ptl promoter | This study |