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Electron micrograph of the intracellular inclusions induced by GFP-apoE4(Δ272–299) in Neuro-2a cells. Neuro-2a cells were transiently transfected with GFP (a) or GFP-apoE4(Δ272–299) (b and c). GFP-positive cells were sorted with a fluorescence-activated cell sorter, pelleted by centrifugation, and examined with a JEOL CCX-100II electron microscope. (Original magnifications: a and b, ×25,000; c, ×60,000.)
