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Saturation densities of first-round assay started with 105 cells
| Cell count (×105) |
||
| Serum, % | 2 wk | 3 wk |
| 2 | 4.3 ± 0.1 | 4.7 ± 0.1 |
| 5 | 9.6 ± 0.5 | 11.3 ± 0.4 |
| 10 | 21.4 ± 0.5 | 23.0 ± 0.9 |
NIH 3T3 cells were grown to confluence in 2%, 5%, and 10% calf serum in MCDB 402 medium for 2 and 3 wk. Some of the cultures were trypsinized and the cells counted for saturation density.
