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. 2013 Aug 2;110(34):E3152–E3161. doi: 10.1073/pnas.1305269110

Fig. 3.

Fig. 3.

Human breast carcinomas with high Tn expression contain ER-localized GalNAc-Ts activity. (A and B) Tn (VVL) and T (PNA) expression in microarrays of human breast tumor biopsies. H&E staining is shown in B. (Scale bars, 100 µm.) (C and D) Quantification of Tn (C) and T (D) staining in human breast cancer biopsies. *P < 0.01 and **P < 0.05 relative to normal tissue cores (Mann–Whitney). NS, not significant. Histological grade confirmed by pathologist. Grade 1: well differentiated; Grade 2: moderately differentiated; Grade 3: poorly differentiated. (E) Costaining with VVL or PNA and ER-marker Calnexin in human breast tumor biopsies. Costaining of Calnexin and ER-protein Calreticulin for comparison. (Scale bar, 10 µm.) (F) Quantification of VVL-Calnexin colocalization in 25 randomly acquired malignant high Tn breast cancer cores from the BR2082 array. Nuclei stained using Hoechst.