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. 2013 Aug 2;110(34):E3152–E3161. doi: 10.1073/pnas.1305269110

Fig. 5.

Fig. 5.

Surface Tn-modified proteins regulate the adhesiveness and motility of 4T1 carcinoma cells containing ER-localized GalNAc-Ts. (A) Cell surface Tn staining (VVL) in 4T1 cells. (Scale bar, 10 µm.) (B) Costaining Tn (VVL) with the lamellipodia marker Tks5/FISH in 4T1 cells. (Scale bar, 10 µm.) (C) Adhesion of 4T1 cells to fibronectin after VVL preincubation for 20 min and quantification of VVL bound to cells after 20-min incubation. *P < 0.01 relative to untreated cells. (D) Wound healing of 4T1 cells on fibronectin after VVL preincubation for 20 min. (Scale bars, 100 µm.) (E) Mean of wound closure ± SEM (n = 3 experiments). *P < 0.01 relative to PNA treated cells or stated samples. (Scale bars, 10 µm.) Nuclei stained using Hoechst.