Table 1.
HO expression profile in WT and mutant cells
| Mother |
Daughter |
|||||||
| Strain | Pon, % | N | Amplitude (on)* | CV† | Pon, % | N | Amplitude (on)* | CV† |
| WT | 98.0 ± 1.1 | 154 | 1 ± 0.02 | 0.31 | 2.3 ± 1.3 | 131 | NA | 1.36 |
| swi5 | 2.7 ± 0.9 | 295 | NA | 2.40 | 6.3 ± 1.6 | 222 | 1.18 ± 0.11 | 2.49 |
| ash1 | 98.4 ± 1.1 | 127 | 1.0 ± 0.02 | 0.27 | 93.5 ± 3.1 | 62 | 1.11 ± 0.06 | 0.41 |
| swi5ash1 | 2.7 ± 0.8 | 408 | 0.86 ± 0.09 | 1.68 | 33.7 ± 2.5 | 356 | 1.13 ± 0.04 | 1.33 |
| swi5ace2 | ∼1 | ∼490 | NA | NA | ∼1 | ∼490 | NA | NA |
| swi5ash1ace2 | ∼1 | ∼440 | NA | NA | ∼1 | ∼440 | NA | NA |
| gcn5 | 32.3 ± 1.9 | 610 | 0.95 ± 0.03 | 1.42 | 3.4 ± 1.2 | 232 | NA | 1.85 |
| swi2-314 | 18.5 ± 1.8 | 453 | 0.84 ± 0.04 | 1.80 | 0 | 168 | NA | 0.66 |
| pob3, 30C | 78.6 ± 3.3 | 159 | 0.70 ± 0.02 | 0.59 | 1.9 ± 1.3 | 106 | NA | 0.76 |
| spt16-11, 30C | 71.6 ± 5.0 | 81 | 0.92 ± 0.05 | 0.74 | 7.4 ± 3.0 | 81 | NA | 1.17 |
| spt16-11, 32C | 58.1 ± 5.1 | 93 | 0.87 ± 0.05 | 0.87 | 6.5 ± 2.8 | 77 | NA | 1.49 |
| rpd3 | 99.1 ± 0.9 | 114 | 1.0 ± 0.03 | 0.32 | 53.8 ± 5.2 | 91 | 1.20 ± 0.06 | 0.90 |
| swi5rpd3 | 39.5 ± 3.2 | 228 | 0.99 ± 0.05 | 1.16 | 68.2 ± 3.8 | 151 | 1.29 ± 0.05 | 0.82 |
| swi5rpd3ace2 | 34.6 ± 4.2 | 130 | 1.09 ± 0.07 | 1.30 | 20.0 ± 3.7 | 115 | 1.09 ± 0.15 | 1.83 |
| gal11 | 92.5 ± 2.7 | 93 | 0.41 ± 0.01 | 0.28 | 16.9 ± 4.4 | 71 | 0.44 ± 0.03 | 0.94 |
| swi6 | 43.3 ± 9.0 | 30 | 0.56 ± 0.04 | 0.86 | 0 | 27 | NA | 0.67 |
*
Average expression amplitude during the “on” cell cycle normalized by that in the WT mother cells. The amplitude is listed as NA when the sample size is too small (on-cycle count <10). Error bar represents SE (same as in the Pon). NA, not available.
†
Coefficient of variance (CV) of the expression amplitude among all cell cycles, including on and off, defined as the ratio of the SD to the mean.