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. Author manuscript; available in PMC: 2014 Jul 17.
Published in final edited form as: Cell Host Microbe. 2013 Jul 17;14(1):26–37. doi: 10.1016/j.chom.2013.06.007

Figure 3. Growth of E. coli Nissle 1917 strains in iron-rich and iron-limited media.

Figure 3

Growth of E. coli Nissle wild-type and the mutants in iron uptake tonB or iroN fuyA iutA chuA (Δ4) was determined. (A,C,E) Growth of E. coli Nissle wild-type and the tonB mutant in DMEM/F12 (A) or DMEM/F12 supplemented with 10% fetal bovine serum (C) or nutrient broth (NB) supplemented with Dipyridyl (E). (B,D,F) Growth of E. coli Nissle wild-type and the Δ4 mutant in DMEM/F12 (B) or DMEM/F12 supplemented with 10% fetal bovine serum with the absence (D) or presence (F) of 1μg/ml lipocalin-2 (Lcn2). Bacteria were enumerated at 2h, 5h, and 8h after inoculation. Bars represent the geometric means ± standard deviation of at least three experiments. STM=S. Typhimurium; EcN=E. coli Nissle. Significant differences are indicated by * (P value ≤ 0.05) or ** (P value ≤ 0.01). (See also Figure S2 and Table S2)