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. Author manuscript; available in PMC: 2015 Jan 30.
Published in final edited form as: J Neurosci Methods. 2013 Nov 8;222:82–90. doi: 10.1016/j.jneumeth.2013.10.014

Figure 5. Functional connectivity of patterned neuronal populations in the circuit.

Figure 5

(A) Diagram of experimental set up. Flow of extracellular solution in the recording chamber (∼5 mls/min) was perpendicular to the axis of the axonal tracts Extracellular solution flowed through the first two tubes and GABA flowed through the third barrel. The opening to the first barrel is positioned above the patterned cortical neurons from the upper microwell. AP currents were recorded from cortical neurons in the lower microwell. (B) Example of action potential currents recorded from cortical neurons. Application of 10 μM GABA resulted in a significant increase in AP firing frequency. (C) Average frequency of AP currents recorded from neurons before and after application of 10 μM GABA (n = 5/group; *, p = 0.03).