(a) DIV28 cultured cortical neurons expressing GFP were treated with vehicle or with NRG1β (5nM) for 2 days. Neurons were fixed and immunostained for GFP.
(b) High magnification images of dendrites for control and NRG1-treated neurons. Scale bar=10µm.
(c) Quantification of the effects of NRG1 on spine morphology. NRG1 increases spine area and density. N=19 cells, 1300 spines for vehicle; 17 cell, 1416 spines for NRG1. Data are the mean±SEM. **p<0.01, ***p<0.001.