(a) Cultured cortical neurons generated from WT or KALRN KO mice were transfected with either GFP alone or GFP plus a plasmid expressing erbB4 for 3-days. Scale bar=10µm.
(b) Quantification of cells in a. ErbB4 overexpression increased spine area in WT, but not KO neurons, without affecting spine density. N=8 cells, 423 spines for WT, 8 cells, 454 spines for WT+erbB4; 8 cells, 344 spines for KO; 8 cells, 336 spines for KO+erbB4. Data are the mean±SEM. ***p<0.001.