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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: Cancer Res. 2013 Jun 6;73(15):4697–4710. doi: 10.1158/0008-5472.CAN-13-0930

Figure 6. Stromal PR is diminished in PTENKO/Kras progesterone resistant tumors by methylation.

Figure 6

(A) Abundant PR was detected in the epithelium and stroma of PTENKO tumors (a). Diminution in stromal PR was detected in PTENKO/Kras tumors (b). (B) Western blot confirmed a decrease in stromal PR of PTENKO/Kras tumors. ERK was a loading control. (C) Q-PCR revealed a significant decrease in PR-A+ PR-B transcript in tumor stroma of PTENKO/Kras compared to PTENKO tumors (a). A non-significant decrease in levels of PR-B transcript was detected (b). (D) Schematic of the PR-A promoter with CpG islands is shown. Based on bisulfite sequencing, a higher percentage of the PR-A promoter CpG islands were methylated in tumor stroma of PTENKO/Kras compared to PTENKO tumors. Three independent tumors for each group were analyzed. Scale bars equal 50 μm.