Table 1.
GO terms are overrepresented in the skin of AEW-treated mice in a TRPA1- and scratch-dependent manner
| GO term | GO ID | n | % | p-value |
|---|---|---|---|---|
| Neuronal growth and development | ||||
| Neurogenesisa | 22008 | 49 | 7.2 | 4.96E-11 |
| Neuron differentiationa | 30182 | 35 | 7.6 | 9.32E-09 |
| Neuron projection developmenta | 31175 | 25 | 9.1 | 4.01E-08 |
| Regulation of neuron differentiationa | 45664 | 22 | 10.8 | 1.22E-08 |
| Neuron developmenta | 48666 | 29 | 8.1 | 3.84E-08 |
| Generation of neuronsa | 48699 | 47 | 7.4 | 6.17E-11 |
| Neuron projection morphogenesisa | 48812 | 21 | 9.8 | 1.34E-07 |
| Regulation of neurogenesisa | 50767 | 24 | 9.5 | 3.08E-08 |
| Regulation of axon extensionb | 30516 | 8 | 26.7 | 5.66E-07 |
| Regulation of axon extensionb | 30516 | 8 | 26.7 | 5.66E-07 |
| Negative regulation of axon extensionb | 30517 | 5 | 41.7 | 6.89E-06 |
| Negative regulation of axon extensionb | 30517 | 5 | 41.7 | 6.89E-06 |
| Regulation of axon extension involved in axon guidanceb | 48841 | 5 | 55.6 | 1.17E-06 |
| Regulation of axonogenesisb | 50770 | 12 | 19.0 | 5.00E-08 |
| Transport and ion homeostasis | ||||
| Transporta | 6810 | 253 | 10.9 | 7.43E-96 |
| Regulation of transporta | 51049 | 53 | 10.3 | 4.01E-18 |
| Positive regulation of transporta | 51050 | 19 | 8.3 | 6.42E-06 |
| Water transportc | 6833 | 6 | 40.0 | 1.04E-06 |
| Ion transportb | 6811 | 151 | 21.0 | 3.47E-95 |
| Cation transportb | 6812 | 114 | 21.0 | 3.68E-71 |
| Potassium ion transportb | 6813 | 36 | 23.7 | 8.36E-25 |
| Sodium ion transportb | 6814 | 43 | 32.6 | 7.74E-36 |
| Calcium ion transportb | 6816 | 22 | 16.9 | 1.71E-12 |
| Anion transportb | 6820 | 42 | 29.6 | 4.59E-33 |
| Chloride transportb | 6821 | 18 | 27.7 | 2.30E-14 |
| Zinc ion transportb | 6829 | 10 | 40.0 | 2.28E-10 |
| Neurotransmitter transportb | 6836 | 14 | 16.3 | 3.18E-08 |
| Amino acid transportb | 6865 | 30 | 36.6 | 4.10E-27 |
| Cellular ion homeostasis | 6873 | 38 | 10.9 | 5.03E-14 |
| Cellular calcium ion homeostasisb | 6874 | 20 | 12.2 | 6.87E-09 |
| Regulation of transporter activityb | 32409 | 9 | 20.0 | 1.55E-06 |
| Regulation of ion transmembrane transporter activityb | 32412 | 8 | 18.6 | 1.05E-05 |
| Regulation of calcium ion transport by v-gated calcium channelb | 42391 | 15 | 10.6 | 3.00E-06 |
| Regulation of ion transportb | 43269 | 31 | 17.9 | 8.81E-18 |
| Negative regulation of transportb | 51051 | 16 | 12.6 | 1.39E-07 |
| Cytosolic calcium ion homeostasisb | 51480 | 17 | 15.9 | 1.58E-09 |
| Regulation of calcium ion transportb | 51924 | 12 | 15.0 | 7.63E-07 |
| Regulation of calcium ion transport by v-gated calcium channelb | 51925 | 8 | 22.9 | 2.04E-06 |
| Metal ion homeostasisb | 55065 | 20 | 10.9 | 4.48E-08 |
| Di-, tri-valent inorganic cation homeostasisb | 55066 | 23 | 10.7 | 6.68E-09 |
| Monovalent inorganic cation homeostasisb | 55067 | 8 | 20.5 | 4.87E-06 |
| Calcium ion homeostasisb | 55074 | 20 | 11.9 | 1.04E-08 |
| Calcium ion transmembrane transportb | 70588 | 12 | 22.2 | 7.91E-09 |
| Divalent metal ion transportb | 70838 | 24 | 17.1 | 1.25E-13 |
| Sodium ion exportb | 71436 | 14 | 38.9 | 8.51E-14 |
| Zinc ion transmembrane transportb | 71577 | 6 | 50.0 | 2.05E-07 |
| Epithelial growth and development | ||||
| Response to woundinga | 9611 | 64 | 17.3 | 1.86E-34 |
| Epidermal cell differentiationa | 9913 | 12 | 24.5 | 2.39E-09 |
| Keratinocyte differentiationa | 30216 | 11 | 24.4 | 1.14E-08 |
| Epithelial cell differentiationa | 30855 | 20 | 14.7 | 2.42E-10 |
| Wound healinga | 42060 | 21 | 16.2 | 1.38E-11 |
| Epithelium developmenta | 60429 | 41 | 12.2 | 7.45E-17 |
| Morphogenesis of a branching epitheliuma | 61138 | 16 | 13.9 | 3.37E-08 |
| Epidermis developmentb | 8544 | 25 | 19.2 | 2.38E-15 |
| Keratinizationb | 31424 | 7 | 28.0 | 2.05E-06 |
| Regulation of epithelial cell proliferationb | 50678 | 13 | 16.9 | 6.29E-08 |
| Positive regulation of epithelial cell proliferationb | 50679 | 8 | 20.5 | 4.87E-06 |
| Prostanoid process | ||||
| Prostaglandin biosynthetic processb | 1516 | 5 | 45.5 | 4.11E-06 |
| Prostanoid metabolic processb | 6692 | 6 | 42.9 | 6.39E-07 |
| Prostaglandin metabolic processb | 6693 | 6 | 42.9 | 6.39E-07 |
| Prostanoid biosynthetic processb | 46457 | 5 | 45.5 | 4.11E-06 |
| Synapse | ||||
| Synaptic transmissionb | 7268 | 21 | 8.8 | 9.03E-07 |
| Synapse organizationb | 50808 | 9 | 16.7 | 7.60E-06 |
| Regulation of secretionb | 51046 | 20 | 8.2 | 4.44E-06 |
| Negative regulation of secretionb | 51048 | 10 | 14.3 | 9.96E-06 |
| Stimulus response | ||||
| Regulation of response to external stimulusa | 32101 | 21 | 12.3 | 2.54E-09 |
| Response to chemical stimulusa | 42221 | 88 | 8.1 | 4.17E-22 |
| Regulation of response to stimulusa | 48583 | 53 | 12.3 | 1.73E-21 |
| Positive regulation of response to stimulusa | 48584 | 31 | 13.7 | 2.01E-14 |
| Negative regulation of response to stimulusa | 48585 | 13 | 11.7 | 4.69E-06 |
| Response to stimulusa | 50896 | 182 | 7.2 | 1.11E-39 |
| Positive regulation of response to external stimulusb | 32103 | 15 | 20.8 | 2.82E-10 |
| Immune process | ||||
| Immune response | 6955 | 54 | 15.4 | 1.14E-26 |
| Regulation of cytokine productiona | 1817 | 19 | 12.6 | 9.70E-09 |
| Positive regulation of cytokine productiona | 1819 | 11 | 14.9 | 2.41E-06 |
| Immune system processa | 2376 | 86 | 12.3 | 2.10E-34 |
| Immune system developmenta | 2520 | 26 | 8.6 | 6.05E-08 |
| Regulation of immune system processa | 2682 | 49 | 13.1 | 3.48E-21 |
| Positive regulation of immune system processa | 2684 | 38 | 15.8 | 1.71E-19 |
| Defense responsea | 6952 | 58 | 13.1 | 6.46E-25 |
| Inflammatory responsea | 6954 | 43 | 18.9 | 3.52E-25 |
| Cytokine-mediated signaling pathwaya | 19221 | 21 | 24.1 | 3.30E-15 |
| Innate immune responsea | 45087 | 17 | 14.2 | 9.56E-09 |
| Leukocyte activationa | 45321 | 23 | 9.8 | 3.31E-08 |
| Regulation of inflammatory responsea | 50727 | 16 | 16.2 | 3.70E-09 |
| Regulation of immune responsea | 50776 | 31 | 14.0 | 1.07E-14 |
| Lymphocyte activationc | 46649 | 21 | 10.8 | 2.68E-08 |
| Regulation of cell activationc | 50865 | 26 | 14.6 | 5.82E-13 |
| Regulation of leukocyte proliferationc | 70663 | 18 | 19.1 | 2.10E-11 |
| Activation of immune responseb | 2253 | 12 | 12.6 | 4.91E-06 |
| Regulation of acute inflammatory responseb | 2673 | 10 | 26.3 | 2.45E-08 |
| Positive regulation of acute inflammatory responseb | 2675 | 7 | 30.4 | 1.09E-06 |
| Negative regulation of immune system processb | 2683 | 13 | 14.6 | 3.64E-07 |
| Regulation of leukocyte activationb | 2694 | 26 | 15.1 | 2.56E-13 |
| Positive regulation of leukocyte activationb | 2696 | 22 | 19.6 | 7.15E-14 |
| Regulation of immune effector processb | 2697 | 19 | 17.1 | 4.57E-11 |
| Negative regulation of immune effector processb | 2698 | 7 | 25.9 | 3.62E-06 |
| Regulation of production of molecular mediator of immune responseb | 2700 | 10 | 25.0 | 4.21E-08 |
| Immune response-activating signal transductionb | 2757 | 10 | 16.4 | 2.78E-06 |
| Immune response-regulating signaling pathwayb | 2764 | 12 | 18.5 | 7.21E-08 |
| Regulation of adaptive immune responseb | 2819 | 12 | 18.8 | 6.01E-08 |
| T-cell differentiationb | 30217 | 12 | 15.2 | 6.64E-07 |
| Leukocyte chemotaxisb | 30595 | 10 | 31.3 | 3.84E-09 |
| Regulation of B-cell proliferationb | 30888 | 14 | 38.9 | 8.51E-14 |
| Positive regulation of B-cell proliferationb | 30890 | 13 | 48.1 | 2.19E-14 |
| Tumor necrosis factor-mediated signaling pathwayb | 33209 | 5 | 45.5 | 4.11E-06 |
| T-cell activationb | 42110 | 16 | 13.2 | 7.00E-08 |
| Regulation of T-cell differentiationb | 45580 | 11 | 22.0 | 3.73E-08 |
| Positive regulation of T-cell differentiationb | 45582 | 10 | 28.6 | 1.02E-08 |
| Regulation of lymphocyte differentiationb | 45619 | 11 | 18.6 | 2.28E-07 |
| Positive regulation of lymphocyte differentiationb | 45621 | 10 | 27.0 | 1.85E-08 |
| Regulation of lymphocyte proliferationb | 50670 | 17 | 18.5 | 1.37E-10 |
| Positive regulation of lymphocyte proliferationb | 50671 | 14 | 23.3 | 2.22E-10 |
| Positive regulation of inflammatory responseb | 50729 | 12 | 25.5 | 1.42E-09 |
| Positive regulation of immune responseb | 50778 | 19 | 12.8 | 7.76E-09 |
| Regulation of T-cell activationb | 50863 | 16 | 14.3 | 2.30E-08 |
| Regulation of B-cell activationb | 50864 | 15 | 25.9 | 1.01E-11 |
| Positive regulation of T-cell activationb | 50870 | 14 | 19.7 | 2.39E-09 |
| Positive regulation of B-cell activationb | 50871 | 14 | 34.1 | 7.03E-13 |
| Leukocyte migrationb | 50900 | 12 | 25.0 | 1.84E-09 |
| Regulation of lymphocyte activationb | 51249 | 25 | 16.1 | 1.63E-13 |
| Positive regulation of lymphocyte activationb | 51251 | 22 | 21.0 | 1.74E-14 |
| Positive regulation of leukocyte proliferationb | 70665 | 15 | 24.2 | 2.87E-11 |
A number of GO terms are overrepresented in the WT AEW-treated mice relative to WT VEH-treated mice. GO terms are listed with corresponding p-values.
aScratch-dependent only (genes that are not significant in “no scratch” AEW- versus VEH-treated mice).
bTRPA1- and scratch-dependent (genes that are not significant in Trpa1−/− AEW- versus VEH-treated mice and no scratch AEW- versus VEH-treated mice).
cTRPA1-dependent only (genes that are not significant in Trpa1−/− AEW- versus VEH-treated mice).