Figure 10. The absence of AC6 impairs the response to secretagogues in pancreatic fluid secretion in isolated duct fragments and in anaesthetized mice.

A, an expansion in the lumen of sealed isolated duct fragments was observed in WT mice upon VIP (10 nm) stimulation while no change in lumen area was observed in duct from AC6^−/− mice. n = 8–9 duct fragments in each group. B, stimulation by VIP (0.5 μg kg⁻¹, i.v.) or secretin (2 U kg⁻¹, i.v.) increased pancreatic fluid secretion in anaesthetized WT mice but not in AC6^−/− mice. Values are means ± SEM; n = 3–4 mice. *P < 0.05, **P < 0.01 vs. control. †P < 0.05 vs. WT mice.