Table 1.
Pancreas | Acini | Ducts | |
---|---|---|---|
Amylase | 13.78 ± 0.41 | 13.02 ± 0.45 | 24.3 ± 0.64** |
Insulin | 23.04 ± 0.66 | 28.49 ± 0.53** | 32.79 ± 1.62** |
Keratin 19 | 37.11 ± 0.79 | not detected | 33.86 ± 0.52* |
AC3 | 31.38 ± 0.38 | 30.15 ± 0.02 | 31.30 ± 0.19 |
AC4 | 31.41 ± 0.46 | 30.7 ± 1.00 | 26.63 ± 0.63* |
AC6 | 29.39 ± 0.72 | 25.95 ± 0.02* | 26.33 ± 0.79* |
AC7 | 34.57 ± 0.41 | not detected | 32.13 ± 0.38* |
AC9 | 29.53 ± 0.75 | 30.02 ± 1.37 | 30.85 ± 0.95 |
β-Actin | 26.28 ± 0.20 | 27.70 ± 0.62 | 25.06 ± 0.43 |
Real-time quantitative PCR for amylase, insulin, keratin 19 and AC isoform expression in the intact pancreas, isolated pancreatic acini and isolated duct fragments. Data are expressed as mean cycle threshold (CT) values ± SEM from 3–6 mice. CT values that are statistically significant vs. pancreas are highlighted in bold. *P < 0.05 and **P < 0.01 vs. pancreas.