Table 1.
Primers for qRT-PCR and TaqMan low density array (LDA) assay ID
| A. Primers for qRT-PCR using SYBR Green assay | ||
|---|---|---|
| Gene products | Sense primer | Antisense primer |
| MGF | GCCCCCATCTACCAACAAGAACAC | CGGTGGCATGTCACTCTTCACTC |
| IGF-1Ea | GACATGCCCAAGACCCAGAAGGA | CGGTGGCATGTCACTCTTCACTC |
| GAPDH | CCTCCTGCACCACCAACTGCTT | GAGGGGCCATCCACAGTCTTCT |
| RPLP0 | GGAAACTCTGCATTCTCGCTTCCT | CCAGGACTCGTTTGTACCCGTTG |
| B. Applied Biosystems TaqMan Low Density Array assay ID | ||
|---|---|---|
| Gene products | Assay ID | |
| MyoD | Hs02330075_g1 | |
| Myogenin | Hs01072232_m1 | |
| HGF | Hs00300159_m1 | |
| c-Met | Hs01565581_m1 | |
| FGF2 | Hs00266645_m1 | |
| FGFR1 | Hs01552926_m1 | |
| Pax7 | Hs00242962_m1 | |
| Myostatin | Hs00193363_m1 | |
| CDKN1A (p21) | Hs01121172_m1 | |
| CDKN1B (p27) | Hs00153277_m1 | |
| RPLP0 | Hs99999902_m1 | |
A. mRNA expression of MGF (IGF-1Ec), IGF-1Ea and RPLP0 was analysed by SYBR Green-based quantitative real-time RT-PCR. B. TaqMan-based quantitative real-time RT-PCRs of MyoD1, myogenin, HGF, c-Met, FGF2, FGFR1, Pax7, myostatin, CDKN1A, CDKN1B and RPLP0 mRNA were performed using ABI TaqMan Low Density Arrays.