TABLE 2.

Sequences of the primers used to detect bla genes and their promoter regions

PCR target	Primer name	Primer sequencea
blaCTX-M (CTX-M-1 group)	M13 upper	5′-GGT TAA AAA ATC ACT GCG TC-3′
	M13 lower	5′-TTG GTG ACG ATT TTA GCC GC-3′
blaCTX-M (CTX-M-2 group)	M25 upper	5′-ATG ATG ACT CAG AGC ATT CG-3′
	M25 lower	5′-TGG GTT ACG ATT TTC GCC GC-3′
blaCTX-M (CTX-M-9 group)	M9 upper	5′-ATG GTG ACA AAG AGA GTG CA-3′
	M9 lower	5′-CCC TTC GGC GAT GAT TCT C-3′
blaTEM	OT3	5′-ATG AGT ATT CAA CAT TTC CG-3′
	OT4	5′-CCA ATG CTT AAT CAG TGA GG-3′
blaCMY	CF1	5′-ATG ATG AAA AAA TCG TTA TGC-3′
	CF2	5′-TTG TAG CTT TTC AAG AAT GCG C-3′
tnpISEcp1b	ISEcp1	5′-AAA AAT GAT TGA AAG GTG GT-3′
	MA1 reverse	5′-ACY TTA CTG GTR CTG CAC AT-3′
tnpIS26	IS26	5′-AGC GGT AAA TCG TGG AGT GA-3′
	MA1 reverse	5′-ACY TTA CTG GTR CTG CAC AT-3′
IS903	IS903 reverse	5′-CGG TTG TAA TCT GTT GTC CA-3′
	M1 IS903	5′-CGT CGG CGG CTA AAA TCG TC-3′
	M9 IS903	5′-CTA CGG CAC CAC CAA TGA TA-3′
ORF513	ORF513	5′-TGG AAG AGG GCG AAG ACG AT-3′
	M2 reverse upper	5′-CGA ATG CTC TGA GTC ATC AT-3′
	M9 reverse upper	5′-TGC ACT CTC TTT GTC ACC AT-3′
ORF1005	ORF1005 reverse	5′-ATC CAT AAT AGC ATC CAT CAT-3′
	M9 reverse lower	5′-GAG AAT CAT CGC CGA AGG G-3′
sul1	sul1 reverse	5′-GCT CAA GAA AAA TCC CAT CCC C-3′
	M2 reverse lower	5′-GCG GCG AAA ATC GTA ACC CA-3′
	M9 reverse lower	5′-GAG AAT CAT CGC CGA AGG G-3′
ORF1	M3 int upp	5′-TCA CCC AGC CTC AAC CTA AG-3′
	ORF1 pol M3	5′-GCA CCG ACA CCC TCA CAC CT-3′

^aY = C or T; R = A or G.

^btnp, transposase.