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hPSCs grown on grooved surfaces (d–i) align well with the direction of the groove and exhibit a change in morphology and proliferation, as compared to control hPSCs grown on flat surfaces (a–c). Neuroepithelium markers are upregulated, while mesendodermal markers are downregulated, as compared to controls. Adapted with permission from Lee et al.67 Color images available online at www.liebertpub.com/teb
