Table 1.

Selectivity Study: Strains Extracted DNAs and Results

Strain	No. of Tested Strains	3-plex Real-Time PCR bont/type				5-plex Real-Time PCR bont/type
		C	D	CD	DC	C	D	CD	DC
Clostridium botulinum type C	9	9	–	–	–	9	–	–	–
Clostridium botulinum type CD	24	24	–	24	–	24	–	22	–
Clostridium botulinum type D	5	–	5	5	–	–	5	5	–
Clostridium botulinum type DC	24	–	24	–	24	–	24	–	24
Clostridium botulinum type A	22	–	–	–	–	–	–	–	–
Clostridium botulinum type A(B)	1	–	–	–	–	–	–	–	–
Clostridium botulinum type Ab	4	–	–	–	–	–	–	–	–
Clostridium botulinum type B	21	–	–	–	–	–	–	–	–
Clostridium botulinum type F	4	–	–	–	–	–	–	–	–
Clostridium butyricum type E	7	–	–	–	–	–	–	–	–
Clostridium butyricum	1	–	–	–	–	–	–	–	–
Clostridium carnis	1	–	–	–	–	–	–	–	–
Clostridium difficile	1	–	–	–	–	–	–	–	–
Clostridium histolyticum	1	–	–	–	–	–	–	–	–
Clostridium novyi	1	–	–	–	–	–	–	–	–
Clostridium paraputrificum	1	–	–	–	–	–	–	–	–
Clostridium perfringens	1	–	–	–	–	–	–	–	–
Clostridium sordelli	1	–	–	–	–	–	–	–	–
Clostridium sporogenes	1	–	–	–	–	–	–	–	–
Bacillus cereus	1	–	–	–	–	–	–	–	–
Bacillus coagulans	1	–	–	–	–	–	–	–	–
Bacillus subtilis	1	–	–	–	–	–	–	–	–
Campylobacter jejuni	1	–	–	–	–	–	–	–	–
Citrobacter fruendii	1	–	–	–	–	–	–	–	–
Cronobacter zakazakii	1	–	–	–	–	–	–	–	–
Enterocuccos faecalis	1	–	–	–	–	–	–	–	–
Escherichia coli	1	–	–	–	–	–	–	–	–
Listeria innocua	1	–	–	–	–	–	–	–	–
Listeria monocytogenes	1	–	–	–	–	–	–	–	–
Micrococcus luteus	1	–	–	–	–	–	–	–	–
Pseudomonas aeruginosa	1	–	–	–	–	–	–	–	–
Rodococcus equi	1	–	–	–	–	–	–	–	–
Salmonella Choleraesuis	1	–	–	–	–	–	–	–	–
Staphylococcus aureus	1	–	–	–	–	–	–	–	–
Streptococcus thermophylus	1	–	–	–	–	–	–	–	–