Table 4.

Primer and Probe Concentration and Reporter Labeling

		Mx3005P				CFX96
		3-plex		5-plex		3-plex		5-plex
Target	Primer/Probe	Conc. (nM)	Rep.	Conc. (nM)	Rep.	Conc. (nM)	Rep.	Conc. (nM)	Rep.
bont/C	Forward	600	–	200	–	300	–	200	–
	Reverse	600	–	200	–	300	–	200	–
	Probe	100	HEX	100	HEX	100	HEX	100	HEX
bont/D	Forward	600	–	300	–	300	–	300	–
	Reverse	600	–	300	–	300	–	300	–
	Probe	100	FAM	100	FAM	100	FAM	100	FAM
bont/CD	Forward	600	–	600	–	300	–	600	–
	Reverse	600	–	600	–	300	–	600	–
	Probe	100	HEX	100	Cy5	100	HEX	100	Cy5
bont/DC	Forward	600	–	600	–	300	–	300	–
	Reverse	600	–	600	–	300	–	300	–
	Probe	100	FAM	300	Alexa350	100	FAM	600	Cy5.5
PC	Forward	600	–	600	–	200	–	600	–
	Reverse	600	–	600	–	200	–	200	–
	Probe	50	ROX	50	ROX	50	ROX	50	ROX