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. 2013 Sep;11(Suppl 1):S207–S214. doi: 10.1089/bsp.2012.0084

Table 5.

LOD, Coefficient of Correlation (R2), Slope of Linearity Curve, PCR Efficiency for Each Target

PCR Scheme Parameter bont/C bont/D bont/CD bont/DC
3-plex  LOD (genomes equivalent) 58 43 58 221
   Coefficient of correlation (R2) 0.999 0.998 0.995 0.995
   Slope of linearity curve −3.24 −3.26 −3.41 −3.33
   PCR efficiency 103.4% 102.4% 96.45% 99.66%
5-plex  LOD (genomes equivalent) 58 43 58 221
   Coefficient of correlation (R2) 0.996 0.999 0.999 0.988
   Slope of linearity curve −3.32 −3.29 −3.41 −3.39
   PCR efficiency 100.1% 101.0% 96.61% 96.88%