Figure 2.

EP2 and EP4 expression in Mtb-infected Mφs. A) At 24 h after infection with H37Ra and H37Rv (MOI 10:1), relative gene expression of EP2 and EP4 was analyzed by quantitative real-time RT-PCR using GAPDH as a reference gene (n=3; mean ± sem). *P < 0.05. B) EP2 and EP4 protein expression measured by Western blot analysis (left panel). GAPDH was used as a loading control (n=3). The intensity of the EP2 and EP4 protein was normalized to that of GAPDH and presented as a fold change relative to uninfected control cells. Values are expressed as means ± sem of 3 independent experiments (right panel).