Figure 6.

Role of EP4 in the activation of the p38 MAPK signaling in H37Ra-infected human Mφs. A) Western blot of p38 MAPK phosphorylation in Mφs stimulated with the EP4 agonist ONO-AE1-329 (10 μM). Mφ lysates treated with DMSO (final concentration: 0.1%) only and with the TLR1/2 agonist Pam3CSK4 (10 μg/ml) were used as negative and positive controls, respectively (n=4). B) Western blot of p38 MAPK phosphorylation of H37Ra (MOI 10:1)-infected Mφs treated with and without the EP4 antagonist AH23848 (10 μM) at 30 min and 240 min after infection (n=4).