Figure 5.

Tropoelastin-induced signaling pathways involve EBP and PKA. Tropoelastin signaling is mediated through the EBP (A–E) and involves the intracellular mediator PKA (F–J). MMP-12 (A, F), CXCL8 (B, G), CXCL5 (C, H), and CXCL1 (D, I) expression was significantly reduced in the presence of lactose, a competitive binding ligand for EBP and H-89, a specific inhibitor of PKA. GAPDH (E, J) was used as a control gene for both EBP and PKA inhibition studies. Results shown are representative of 2 independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001.