Table 1.
Primer sets for quantitative RT-PCR analysis
| Gene | Full name | GeneBank ID | Sequences, forward/reverse 5′–3′ | Amplicon size (bp) |
|---|---|---|---|---|
| CYP7A1 | Cytochrome P450 7A1 | NM_012942 | TGAAAGCGGGAAAGCAAAGACCAC/TCTTGGACGGCAAAGAGTCTTCCA | 117 |
| CYP8B1 | Cytochrome P450 8B1 | NM_031241 | TCCATATGTCCCGGCAGGTTCTTT/TGTCAGGGTCCACCAGTTCAAAGT | 97 |
| CYP27A1 | Cytochrome P450 27A1 | NM_178847 | ACCTTTCCTGAGCTGATCTTGGCT/GCATGTGGGCAAAGTCCTTGTTCT | 169 |
| StAR | Steroidogenic acute regulatory protein | NM_031558 | AAGCCAGCAGGAGAATGGAGATGA/TGACATTTGGGTTCCACTCTCCCA | 158 |
| Fatp2 | Fatty acid transport protein 2 | NM_031736 | AGTACATCGGTGAACTGCTTCGGT/CAATGTTGCCTTCAGTGGAAGCGT | 180 |
| BACS/Fatp5 | Bile acid CoA synthase | NM_024143 | TTCAGGGACCACTGGACTTCCAAA/ACCACATCATCAGCTGTTCTCCCA | 105 |
| BAAT | Bile acid CoA: amino acid N-acyltransferase | NM_017300 | GGTTGGCATCCTTTCTGTGTGCAT/ATTCTTCACTGCAGGGTGTAGGCT | 167 |
| CYP3A11 | Cytochrome P450 3A11 | NM_153312 | TCCAGCTGATGCTGAACGCTCATA/AGGGTGAGTGGCCAGGAAATACAA | 164 |
| SULT2A1 | Sulfotransferase family 2A1 | NM_131903 | TCAGTTCCAAGGCCAAGGTGATCT/AGTTCCCAGTGAGTCTGGCTTCTT | 116 |
| NTCP/Slc10a1 | Na+-taurocholate cotransporting polypeptide | NM_017047 | AGGATGGAGGTGCACAACGTATCA/AGCCCAGTGAGAGCATGATAAGCA | 133 |
| BSEP/Abcb11 | Bile salt export pump | NM_031760 | ATCTGTTAATCCTGGGCAGACGCT/TGGGAGACAATCCCGATGTTGGAA | 180 |
| MRP2/Abcc2 | Multidrug resistance-associated protein 2 | NM_012833 | AACCGGGAAGGTCAAGTTCTCCAT/TTGTCAGAGTCACTGGTCCAAGCA | 153 |
| MRP4/Abcc4 | Multidrug resistance-associated protein 4 | NM_133411 | ATTGTGGGAAGAACTGGAGCTGGA/TGGTTCCAGTGAACAGGACAGGTT | 175 |
| OSTα | Organic solute transporter alpha | NM_001107087 | AGCAATTTCCTTGCTGTGTCCACC/AGGATGACAAGCACCTGGAACAGA | 131 |
| OSTβ | Organic solute transporter beta | XM_238546 | TCCGTTCAGAGGATGCAACTCCTT/CATTCCGTTGTCTTGTGGCTGCTT | 140 |
| ASBT/Slc10a2 | Apical sodium dependent bile acid transporter | NM_017222 | TGGGCTTCCTCTGTCAGTTTGGAA/AGTGTGGAGCAAGTGGTCATGCTA | 196 |
| TGR5/Gpbar1 | G protein-coupled bile acid receptor 1 | NM_177936 | TTGCTCCTGTCAGTCTTGGCCTAT/TTGGGTCTTCCTCGAAGCACTTGT | 191 |
| FGF15 | Fibroblast growth factor 15 | NM_130753 | ACAATTGCCATCAAGGACGTCAGC/TGAAGATGATGTGGAGGTGGTGCT | 172 |
| FGFR4 | Fibroblast growth factor receptor 4 | NM_001109904 | TCACAGTTGAGGCCTTCTGTTCCA/TGCTTCGCTCCTTTGAGGATGAGT | 108 |
| 18s rRNA | 18s ribosomal RNA | NR_046237 | ACGGACCAGAGCGAAAGCAT/TGTCAATCCTGTCCGTGTCC | 120 |