FIG. 2.

Effects of extracellular cations on candidacidal effects of hLf. (A) C. albicans cells were suspended in SPB (•) or PPB (○) (both at pH 7.4) adjusted to different molarities and then incubated with lactoferrin for 2 h. (B) The candidacidal effect of lactoferrin was also tested in 10 mM Tris buffer (pH 7.4) containing either NaCl (•) or KCl (○). (C) Killing activity of lactoferrin against C. albicans cells suspended in 10 mM Tris buffer (pH 7.4) containing different concentrations of CaCl₂ (□) or MgCl₂ (▪). The results are the means ± standard deviations from duplicates of three separate experiments.