FIG. 4.

Effect of lactoferrin on intracellular pH of C. albicans cells. C. albicans cells preloaded with the pH-sensitive fluorescent dye BCECF-AM were exposed to hLf or amphotericin B (AmB) for 2 h, and then the intracellular BCECF-AM fluorescence intensities of treated and nontreated (control [C]) cells were analyzed by flow cytometry. GR-FL, green fluorescence. The micrographs show BCECF- loaded cells incubated in the presence (A) or the absence (B) of lactoferrin and examined by fluorescence microscopy. Bar, 5 μm.